Real-time measurement, analysis, and control in microfluidic systems for personalized medicine and designer materials
- Degree Grantor:
- University of California, Santa Barbara. Materials
- Degree Supervisor:
- Hyongsok T. Soh
- Place of Publication:
- [Santa Barbara, Calif.]
- Publisher:
- University of California, Santa Barbara
- Creation Date:
- 2016
- Issued Date:
- 2016
- Topics:
- Biomedical engineering and Materials science
- Keywords:
- Biosensors,
FACS,
Feedback control,
Microfluidics, and
Aptamer - Genres:
- Online resources and Dissertations, Academic
- Dissertation:
- Ph.D.--University of California, Santa Barbara, 2016
- Description:
The field of microfluidics has enabled the development of powerful tools for analyzing and manipulating phenomena at the micro- and nano-scales, ranging from chemical analysis of biological samples to controlled synthesis of colloidal materials. In this dissertation we explore four unique platforms for real-time microfluidic measurement, analysis, and control systems with applications at the intersection of biomedicine and materials engineering. First, we show that a real-time biosensor can be used to perform closed-loop control of drug concentrations in the bloodstream of live animals. Second, we show that a commercially available cell-sorting instrument can be used to sort heterogeneous suspensions of synthetic microparticles based on shape using optical scattering measurements, resulting in monodisperse microparticle suspensions with well-defined morphology. Third, we report preliminary results for an image-based cell and microparticle sorter capable of sorting objects using two-dimensional high-speed microscopy and real-time image analysis. Finally, we report a contamination-resistant microfluidic assay for quantitative genetic detection based on real-time loop-mediated isothermal amplification, improving the robustness of point-of-care pathogen detection techniques.
- Physical Description:
- 1 online resource (150 pages)
- Format:
- Text
- Collection(s):
- UCSB electronic theses and dissertations
- Other Versions:
- http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:10103618
- ARK:
- ark:/48907/f3qv3mb0
- ISBN:
- 9781339671840
- Catalog System Number:
- 990046534530203776
- Copyright:
- Peter Mage, 2016
- Rights:
- In Copyright
- Copyright Holder:
- Peter Mage
File | Description |
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Access: Public access | |
Mage_ucsb_0035D_12943.pdf | pdf (Portable Document Format) |