Identification and functional analysis of ABI4-interacting proteins in Arabidopsis
- Degree Grantor:
- University of California, Santa Barbara. Molecular, Cellular & Developmental Biology
- Degree Supervisor:
- Ruth R. Finkelstein
- Place of Publication:
- [Santa Barbara, Calif.]
- Publisher:
- University of California, Santa Barbara
- Creation Date:
- 2016
- Issued Date:
- 2016
- Topics:
- Plant sciences and Molecular biology
- Keywords:
- Abiotic stress,
ZFP2,
ABI4,
Arabidopsis,
Abscisic Acid, and
ABA - Genres:
- Online resources and Dissertations, Academic
- Dissertation:
- M.A.--University of California, Santa Barbara, 2016
- Description:
Land plants utilize the hormone abscisic acid (ABA) to cope with drought, salt, and other abiotic stresses. ABA-INSENSITIVE4 (ABI4) is a transcription factor central to the ABA response in Arabidopsis thaliana but there are still many questions surrounding its regulation and transcriptional activity which might be explained by understanding its interacting partners. Here, we present data from a next generation sequencing yeast two-hybrid (NGS-Y2H) screen used to discover ABI4-interacting proteins. Using additional yeast two-hybrid and split-YFP experiments, we were able to confirm an ABI4 interaction with DWA1, DWA2, ZFP2, and eIF3f. Although the E3 ligases DWA1 and DWA2 can physically interact with ABI4, they do not mediate ubiquitination of ABI4. The transcription factor ZFP2 is encoded by a gene that is expressed in root tips of developing embryos and germinating seedlings which is consistent with areas of ABI4 activity. We were unable to determine if ZFP2 is involved in ABA signaling during germination or root growth, but our experiments show that ZFP2 is regulated by auxin and possibly ABA.
- Physical Description:
- 1 online resource (76 pages)
- Format:
- Text
- Collection(s):
- UCSB electronic theses and dissertations
- Other Versions:
- http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:10192227
- ARK:
- ark:/48907/f3bz665j
- ISBN:
- 9781369340426
- Catalog System Number:
- 990047190080203776
- Copyright:
- Kyle Swentowsky, 2016
- Rights:
- In Copyright
- Copyright Holder:
- Kyle Swentowsky
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