Alexandria Digital Research Library

A biochemical linkage between methanogenesis and protein synthesis in hydrogenotrophic archaea

Author:
Oza, Javin Pradip
Degree Grantor:
University of California, Santa Barbara. Biomolecular Science and Engineering
Degree Supervisor:
John Perona
Place of Publication:
[Santa Barbara, Calif.]
Publisher:
University of California, Santa Barbara
Creation Date:
2012
Issued Date:
2012
Topics:
Chemistry, Biochemistry, Biology, Molecular, and Biophysics, General
Keywords:
Methanogenesis
Biochemistry
Archaea
Protein synthesis
Enzyme kinetics
H2-forming methylenetetrahydromethanopterin dehydrogenase
Genres:
Online resources and Dissertations, Academic
Dissertation:
Ph.D.--University of California, Santa Barbara, 2012
Description:

Hydrogenotrophic methanogens possessing the hydrogen-dependent dehydrogenase Hmd also encode paralogs of this protein whose function is poorly understood. Here we present biochemical evidence that the two inactive Hmd paralogs of Methanocaldococcus jannaschii, HmdII and HmdIII, form binary and ternary complexes with several components of the protein translation apparatus. HmdII and HmdIII, but not the active dehydrogenase Hmd, bind with micromolar binding affinities to a number of tRNAs and form ternary complexes with tRNA(Pro) and prolyl-tRNA synthetase (ProRS). Fluorescence spectroscopy experiments also suggest that binding of HmdII and ProRS involves distinct binding determinants on the tRNA. These biochemical data suggest the possibility of a regulatory link between energy production and protein translation pathways that may allow a rapid cellular response to altered environmental conditions.

Physical Description:
1 online resource (214 pages)
Format:
Text
Collection(s):
UCSB electronic theses and dissertations
ARK:
ark:/48907/f3416v00
ISBN:
9781267648686
Catalog System Number:
990038915760203776
Rights:
Inc.icon only.dark In Copyright
Copyright Holder:
Javin Oza
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